Hotspot

rpl14-rps8

Genome · 26924-27548 · 625 bp

Back to Corethrodendron

Pi 0.0016

MarkerSeek score 59.80

Primer available yes

Label rpl14-rps8

Feature Metadata

label_name: rpl14-rps8
feature_type: igs
region: Genome
length_bp: 625
variable_sites: 1
pi: 0.0016
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rps8_p1	GCCCGAGAATTGAGACAGTT	TCACGATGCTTTCTGACGTT	50.0	45.0	57.9	57.9	794-795 bp	1.0	78.2
rpl14-rps8_p2	GCCCGAGAATTGAGACAGTT	TTCACGATGCTTTCTGACGT	50.0	45.0	57.9	57.9	795-796 bp	1.0	78.2
rpl14-rps8_p3	CGCAATAGCCCGAGAATTGA	TCACGATGCTTTCTGACGTT	50.0	45.0	58.4	57.9	801-802 bp	1.0	75.7
rpl14-rps8_p4	CGCAATAGCCCGAGAATTGA	TTCACGATGCTTTCTGACGT	50.0	45.0	58.4	57.9	802-803 bp	1.0	75.7
rpl14-rps8_p5	AGCCCGAGAATTGAGACAGT	TCACGATGCTTTCTGACGTT	50.0	45.0	58.7	57.9	795-796 bp	1.0	73.2