Hotspot

ycf1

SSC · 122462-127663 · 5202 bp

Pi 0.00827

MarkerSeek score 46.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5202
variable_sites: 64
pi: 0.00827
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCTGCGAGGGACTTCTTTAC	ATCCGCCAAGTTTGTCAACT	55.0	45.0	58.0	58.0	530-533 bp	1.0	82.3
ycf1_p2	ATCCACTCGCCATTCTGTTC	ATCCGCCAAGTTTGTCAACT	50.0	45.0	58.0	58.0	596-599 bp	1.0	82.0
ycf1_p3	GATCCACTCGCCATTCTGTT	ATCCGCCAAGTTTGTCAACT	50.0	45.0	58.0	58.0	597-600 bp	1.0	82.0
ycf1_p4	CCTGCGAGGGACTTCTTTAC	ATGCCGTCACCCATTCTTAC	55.0	50.0	58.0	58.0	942-945 bp	1.0	81.7
ycf1_p5	ATCCACTCGCCATTCTGTTC	ATGCCGTCACCCATTCTTAC	50.0	50.0	58.0	58.0	1008-1011 bp	1.0	81.5