Hotspot

rpl32-trnL-UAG

SSC · 112196-112938 · 743 bp

Back to Cordia

Pi 0.00808
MarkerSeek score 62.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
743
variable_sites
9
pi
0.00808
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGTTGTGCGACAAACAAACA TCCTAAGAGCAGCGTGTCTA 40.0 50.0 57.3 57.9 807-812 bp 1.0 74.3
rpl32-trnL-UAG_p2 TGTTGTGCGACAAACAAACA AGCAGCGTGTCTACCAATTT 40.0 45.0 57.3 57.8 800-805 bp 1.0 73.9
rpl32-trnL-UAG_p3 TGTTGTGCGACAAACAAACA CCTAAGAGCAGCGTGTCTAC 40.0 55.0 57.3 57.8 806-811 bp 1.0 73.8
rpl32-trnL-UAG_p4 TGTTGTGCGACAAACAAACA CTAAGAGCAGCGTGTCTACC 40.0 55.0 57.3 57.8 805-810 bp 1.0 73.8
rpl32-trnL-UAG_p5 TGTTGTGCGACAAACAAACA GAGCAGCGTGTCTACCAATT 40.0 50.0 57.3 58.3 801-806 bp 1.0 73.2