Hotspot

trnM-CAU-psaI

LSC · 57800-60247 · 2448 bp

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Pi 0.00955
MarkerSeek score 57.80
Primer available yes
Label trnM-CAU-psaI

Feature Metadata

label_name
trnM-CAU-psaI
feature_type
igs
region
LSC
length_bp
2448
variable_sites
35
pi
0.00955
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-psaI_p1 CCCGCCGTATGAAAGCAATA CCATTGCAATTGCCGGAAAT 50.0 45.0 58.4 58.0 2561-2652 bp 1.0 77.7
trnM-CAU-psaI_p2 CCCGCCGTATGAAAGCAATA AAGAAGCCATTGCAATTGCC 50.0 45.0 58.4 57.9 2567-2658 bp 1.0 77.1
trnM-CAU-psaI_p3 CCGCCGTATGAAAGCAATAC CCATTGCAATTGCCGGAAAT 50.0 45.0 57.3 58.0 2560-2651 bp 1.0 75.7
trnM-CAU-psaI_p4 CCGCCGTATGAAAGCAATAC AAGAAGCCATTGCAATTGCC 50.0 45.0 57.3 57.9 2566-2657 bp 1.0 75.1
trnM-CAU-psaI_p5 TTTGAACCAATGACTCCCGC CCATTGCAATTGCCGGAAAT 50.0 45.0 58.8 58.0 2576-2667 bp 1.0 74.9