Hotspot

psbM-trnD-GUC

LSC · 30562-31262 · 701 bp

Back to Cordia

Pi 0.00573
MarkerSeek score 62.60
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
701
variable_sites
6
pi
0.00573
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGTAGAAAAGCGGTAGGAACT AAATTTTCTGCCAGACCCCC 42.9 50.0 56.4 58.4 833-872 bp 1.0 60.5
psbM-trnD-GUC_p2 AGCGGTAGGAACTAGAATGA AAATTTTCTGCCAGACCCCC 45.0 50.0 55.4 58.4 825-864 bp 1.0 60.4
psbM-trnD-GUC_p3 AGTAGAAAAGCGGTAGGAACT TTCAATTGGTCAGAGCACCG 42.9 50.0 56.4 58.5 794-833 bp 1.0 59.7
psbM-trnD-GUC_p4 AGCGGTAGGAACTAGAATGA TTCAATTGGTCAGAGCACCG 45.0 50.0 55.4 58.5 786-825 bp 1.0 59.6
psbM-trnD-GUC_p5 AGCGGTAGGAACTAGAATGAA AAATTTTCTGCCAGACCCCC 42.9 50.0 56.2 58.4 825-864 bp 1.0 58.8