Hotspot

rpoB-trnC-GCA

LSC · 27028-28308 · 1281 bp

Back to Cordia

Pi 0.00729
MarkerSeek score 51.80
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1281
variable_sites
14
pi
0.00729
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CCGCCTACACAAGCAAATTG CTGGGGGTTTCCGAAAAGAA 50.0 50.0 58.0 58.0 2521-2550 bp 1.0 81.6
rpoB-trnC-GCA_p2 CCGCCTACACAAGCAAATTG TTTCCTATCAGGCTCCCCAA 50.0 50.0 58.0 58.0 2342-2370 bp 1.0 81.5
rpoB-trnC-GCA_p3 CCGCCTACACAAGCAAATTG GGGGAATATTTCGGCGAGTT 50.0 50.0 58.0 58.0 2087-2115 bp 1.0 81.5
rpoB-trnC-GCA_p4 CCGGGGCTTTGCAATATTTG CTGGGGGTTTCCGAAAAGAA 50.0 50.0 58.1 58.0 2209-2238 bp 1.0 81.5
rpoB-trnC-GCA_p5 CCGGGGCTTTGCAATATTTG TTTCCTATCAGGCTCCCCAA 50.0 50.0 58.1 58.0 2030-2058 bp 1.0 81.3