Hotspot

atpH-atpI

LSC · 13672-14828 · 1157 bp

Back to Cordia

Pi 0.00895
MarkerSeek score 65.50
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1157
variable_sites
15
pi
0.00895
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 CTCTACAGCTTGACCTGCAG CGCGGCGTATATAGGTGAAT 55.0 50.0 58.0 57.7 1299-1308 bp 1.0 79.4
atpH-atpI_p2 ATAACGGAAGCGGCAGAAAT CGCGGCGTATATAGGTGAAT 45.0 50.0 58.0 57.7 1220-1229 bp 1.0 79.3
atpH-atpI_p3 GCGATACCCTCTACAGCTTG CGCGGCGTATATAGGTGAAT 55.0 50.0 57.9 57.7 1307-1316 bp 1.0 78.3
atpH-atpI_p4 AATAACGGAAGCGGCAGAAA CGCGGCGTATATAGGTGAAT 45.0 50.0 58.2 57.7 1221-1230 bp 1.0 78.1
atpH-atpI_p5 GCCAATCCAGCAGCAATAAC CGCGGCGTATATAGGTGAAT 50.0 50.0 57.8 57.7 1235-1244 bp 1.0 77.8