Hotspot

rps18

LSC · 71727-72095 · 369 bp

Back to Corchorus

Pi 0.0813

MarkerSeek score 72.10

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 369
variable_sites: 30
pi: 0.0813
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	ACCCTTCCCGAGAGTATGAG	CTTTTTCTTCCCCAACCCGA	55.0	50.0	57.9	58.0	892-969 bp	1.0	83.1
rps18_p2	ACCCTTCCCGAGAGTATGAG	TCTTTTTCTTCCCCAACCCG	55.0	50.0	57.9	58.0	893-970 bp	1.0	83.1
rps18_p3	AAAGGAGGTTCATGGCCAAG	CTTTTTCTTCCCCAACCCGA	50.0	50.0	58.1	58.0	1127-1204 bp	1.0	82.4
rps18_p4	AAAGGAGGTTCATGGCCAAG	TCTTTTTCTTCCCCAACCCG	50.0	50.0	58.1	58.0	1128-1205 bp	1.0	82.4
rps18_p5	AAAGGAGGTTCATGGCCAAG	GGGTTCCCTCGAGAATGAAC	50.0	55.0	58.1	58.0	1248-1327 bp	1.0	82.0