Hotspot

rps11

LSC · 83090-83506 · 417 bp

Back to Corchorus

Pi 0.0408

MarkerSeek score 77.60

Primer available yes

Label rps11

Feature Metadata

label_name: rps11
feature_type: gene
region: LSC
length_bp: 417
variable_sites: 17
pi: 0.0408
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11_p1	TTCCACTGTAGTGTCCGAGT	AGAAGATCTGTTTTGACATGA	50.0	33.3	58.0	52.8	604-622 bp	1.0	42.2
rps11_p2	TTCCACTGTAGTGTCCGAGT	AGAAGATCTGTTTTGACATGAA	50.0	31.8	58.0	53.7	604-622 bp	1.0	42.2
rps11_p3	TTCCACTGTAGTGTCCGAGT	AGAAGATCTGTTTTGACATGAAA	50.0	30.4	58.0	54.5	604-622 bp	1.0	42.2
rps11_p4	TTCCACTGTAGTGTCCGAGT	GAAGATCTGTTTTGACATGAA	50.0	33.3	58.0	52.1	603-621 bp	1.0	42.2
rps11_p5	TTCCACTGTAGTGTCCGAGT	AGAAGATCTGTTTTGACATGAAAT	50.0	29.2	58.0	55.0	604-622 bp	1.0	42.2