Hotspot

rpl20

LSC · 72457-72822 · 366 bp

Back to Corchorus

Pi 0.0427

MarkerSeek score 70.60

Primer available yes

Label rpl20

Feature Metadata

label_name: rpl20
feature_type: gene
region: LSC
length_bp: 366
variable_sites: 15
pi: 0.0427
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20_p1	AGACAATTCCTATTTGAGATAGCCA	CTGAAAGAAAGAATTAGTTATTCATCA	36.0	25.9	57.9	54.6	446 bp	0.5	20.0
rpl20_p2	AGACAATTCCTATTTGAGATAGCCA	ACTGAAAGAAAGAATTAGTTATTCATC	36.0	25.9	57.9	54.6	447 bp	0.5	20.0
rpl20_p3	TCCTATTTGAGATAGCCAAGT	CTGAAAGAAAGAATTAGTTATTCATCA	38.1	25.9	53.7	54.6	439 bp	0.5	20.0
rpl20_p4	TCCTATTTGAGATAGCCAAGT	ACTGAAAGAAAGAATTAGTTATTCATC	38.1	25.9	53.7	54.6	440 bp	0.5	20.0
rpl20_p5	TTCCTATTTGAGATAGCCAAGT	CTGAAAGAAAGAATTAGTTATTCATCA	36.4	25.9	54.6	54.6	440 bp	0.5	20.0