Hotspot

clpP

LSC · 73890-75982 · 2093 bp

Back to Corchorus

Pi 0.0844

MarkerSeek score 80.50

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2093
variable_sites: 164
pi: 0.0844
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGACCCTGTTAGTCCGTTCT	CGCAATGGGGGTTGATTCTA	50.0	50.0	58.0	57.9	2931-2991 bp	1.0	89.8
clpP_p2	CTAGTACATGTTCCTCGGCG	CGCAATGGGGGTTGATTCTA	55.0	50.0	57.9	57.9	2626-2686 bp	1.0	89.5
clpP_p3	AGAGGGCAAAGAGGTAGAGG	TGCCCCCTGTAATACTCCAA	55.0	50.0	58.1	58.0	2892 bp	0.5	58.8
clpP_p4	AGAGGTAGAGGGCAAAGAGG	TGCCCCCTGTAATACTCCAA	55.0	50.0	58.1	58.0	2898 bp	0.5	58.8
clpP_p5	AGAGGGCAAAGAGGTAGAGG	CTGTAATACTCCAACCGCCC	55.0	55.0	58.1	58.1	2886 bp	0.5	58.6