Hotspot

trnI-CAU-rpl23

IRa · 158729-159210 · 482 bp

Back to Corchorus

Pi 0.133
MarkerSeek score 80.90
Primer available yes
Label trnI-CAU-rpl23

Feature Metadata

label_name
trnI-CAU-rpl23
feature_type
igs
region
IRa
length_bp
482
variable_sites
52
pi
0.133
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.81
markerseek_score
80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU-rpl23_p1 TGGCGAATTCGTAGGTTCAA ACCCAATGCTTTATCTCTGTCC 45.0 45.5 57.5 58.1 566-633 bp 1.0 70.4
trnI-CAU-rpl23_p2 TTGGCGAATTCGTAGGTTCA ACCCAATGCTTTATCTCTGTCC 45.0 45.5 57.5 58.1 567-634 bp 1.0 70.4
trnI-CAU-rpl23_p3 TGGCGAATTCGTAGGTTCAA CCCAATGCTTTATCTCTGTCCT 45.0 45.5 57.5 57.8 565-632 bp 1.0 70.0
trnI-CAU-rpl23_p4 TTGGCGAATTCGTAGGTTCA CCCAATGCTTTATCTCTGTCCT 45.0 45.5 57.5 57.8 566-633 bp 1.0 70.0
trnI-CAU-rpl23_p5 TGGCGAATTCGTAGGTTCAA TCGACCCAATGCTTTATCTCT 45.0 42.9 57.5 56.8 569-636 bp 1.0 69.7