Hotspot

trnI-CAU-ycf2

IRb · 91050-91192 · 143 bp

Back to Corchorus

Pi 0.157

MarkerSeek score 74.80

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 143
variable_sites: 19
pi: 0.157
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GGTCCCACTCTTCTACCCTT	GTTGAACCCTGTGAATTGCG	55.0	50.0	58.0	57.9	1020-1111 bp	1.0	89.6
trnI-CAU-ycf2_p2	GGTCCCACTCTTCTACCCTT	CCAGCGACGGTTTCATTAGA	55.0	50.0	58.0	58.0	1410-1501 bp	1.0	89.1
trnI-CAU-ycf2_p3	GGTCCCACTCTTCTACCCTT	TCCAGCGACGGTTTCATTAG	55.0	50.0	58.0	58.0	1411-1502 bp	1.0	89.1
trnI-CAU-ycf2_p4	GAAGAGTTCGACCCAATGCT	CCAGCGACGGTTTCATTAGA	50.0	50.0	57.9	58.0	1345-1436 bp	1.0	89.0
trnI-CAU-ycf2_p5	GGTCCCACTCTTCTACCCTT	AATCATGGTCCTTGCGGATC	55.0	50.0	58.0	58.0	1502-1593 bp	1.0	88.7