Hotspot

trnK-UUU-rps16

LSC · 4379-5273 · 895 bp

Back to Corchorus

Pi 0.0157

MarkerSeek score 71.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 895
variable_sites: 13
pi: 0.0157
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CTGCCGATGGTATGAACGAA	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.1	58.0	1026-1087 bp	1.0	84.4
trnK-UUU-rps16_p2	TGGTCTTACAAACTCTGCCG	AAAAGGAGCTCAACCCACAG	50.0	50.0	57.8	58.0	1040-1101 bp	1.0	83.6
trnK-UUU-rps16_p3	AAAGCCGAGTACTCTACCGT	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.2	58.0	962-1023 bp	1.0	83.6
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.2	58.0	961-1022 bp	1.0	83.6
trnK-UUU-rps16_p5	CCGCACTTAAAAGCCGAGTA	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.3	58.0	971-1032 bp	1.0	82.7