Hotspot

ycf1

SSC · 129127-134808 · 5682 bp

Back to Corchoropsis

Pi 0.00329

MarkerSeek score 41.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5682
variable_sites: 28
pi: 0.00329
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGTTTGATTGTTGGGATTAGT	TCACGAATTTGAACAGACAA	33.3	35.0	53.3	53.4	181 bp	1.0	41.8
ycf1_p2	TGTTTGATTGTTGGGATTAGTT	TCACGAATTTGAACAGACAA	31.8	35.0	54.2	53.4	181 bp	1.0	41.8
ycf1_p3	TTGTTTGATTGTTGGGATTAGT	TCACGAATTTGAACAGACAA	31.8	35.0	54.2	53.4	182 bp	1.0	41.8
ycf1_p4	TGTTTGATTGTTGGGATTAGT	ATCACGAATTTGAACAGACA	33.3	35.0	53.3	53.2	182 bp	1.0	41.8
ycf1_p5	TTGTTTGATTGTTGGGATTAGTT	TCACGAATTTGAACAGACAA	30.4	35.0	55.0	53.4	182 bp	1.0	41.8