Hotspot

ndhD

SSC · 120815-122344 · 1530 bp

Back to Corchoropsis

Pi 0.00174

MarkerSeek score 32.70

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1530
variable_sites: 4
pi: 0.00174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GTAAATTCCGCACTTGTGGC	TGTCGGTTGTAAGAGATGCG	50.0	50.0	58.0	58.0	2164-2175 bp	1.0	80.4
ndhD_p2	GTAAATTCCGCACTTGTGGC	CAAATTGCTTCCGCTCCAAG	50.0	50.0	58.0	58.0	2196-2207 bp	1.0	80.3
ndhD_p3	TAAATTCCGCACTTGTGGCT	TGTCGGTTGTAAGAGATGCG	45.0	50.0	58.1	58.0	2163-2174 bp	1.0	79.8
ndhD_p4	TAAATTCCGCACTTGTGGCT	CAAATTGCTTCCGCTCCAAG	45.0	50.0	58.1	58.0	2195-2206 bp	1.0	79.7
ndhD_p5	GTAAATTCCGCACTTGTGGC	AATTGCTTCCGCTCCAAGAA	50.0	45.0	58.0	58.1	2194-2205 bp	1.0	79.7