Hotspot

clpP

LSC · 74132-76297 · 2166 bp

Back to Corchoropsis

Pi 0.00216

MarkerSeek score 59.20

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2166
variable_sites: 7
pi: 0.00216
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGGATTGGTGTTAAATCGTTGC	ACGGCCAATATTGTTAAGACCA	40.9	40.9	57.8	58.0	2271-2279 bp	1.0	46.9
clpP_p2	GGATTGGTGTTAAATCGTTGCT	ACGGCCAATATTGTTAAGACCA	40.9	40.9	57.6	58.0	2270-2278 bp	1.0	44.7
clpP_p3	TGGATTGGTGTTAAATCGTTGC	ACGTTTCCACATCAAAGTGA	40.9	40.0	57.8	55.5	2244-2252 bp	1.0	43.4
clpP_p4	TGGATTGGTGTTAAATCGTTGC	ACGTTTCCACATCAAAGTGAA	40.9	38.1	57.8	56.3	2244-2252 bp	1.0	41.7
clpP_p5	TGGATTGGTGTTAAATCGTTGC	CGGCCAATATTGTTAAGACCA	40.9	42.9	57.8	56.3	2270-2278 bp	1.0	41.7