Hotspot

ndhF-rpl32

SSC · 116603-117942 · 1340 bp

Back to Corchoropsis

Pi 0.00996

MarkerSeek score 65.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1340
variable_sites: 20
pi: 0.00996
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCCGTCGGAAAAAGTA	AAAAGCTTTCAATGCTGCCC	45.0	45.0	58.0	57.8	1535-1539 bp	1.0	79.7
ndhF-rpl32_p2	TTGGAAGTGGCACAAAAGGT	AAAAGCTTTCAATGCTGCCC	45.0	45.0	58.1	57.8	1488-1492 bp	1.0	79.0
ndhF-rpl32_p3	TTTGTTGCCGTCGGAAAAAG	AAAAGCTTTCAATGCTGCCC	45.0	45.0	57.8	57.8	1537-1541 bp	1.0	78.5
ndhF-rpl32_p4	AGGAATTGGAAGTGGCACAA	AAAAGCTTTCAATGCTGCCC	45.0	45.0	57.6	57.8	1493-1497 bp	1.0	77.1
ndhF-rpl32_p5	TTGCCGTCGGAAAAAGTAGG	AAAAGCTTTCAATGCTGCCC	50.0	45.0	58.5	57.8	1533-1537 bp	1.0	76.2