Hotspot

rpoB-trnC-GCA

LSC · 27276-28666 · 1391 bp

Back to Corchoropsis

Pi 0.0024

MarkerSeek score 63.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1391
variable_sites: 5
pi: 0.0024
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCCATTTTCATCTCCGAGCA	CGGGTTTTTGTTGATCAGGC	50.0	50.0	58.0	57.9	1477-1500 bp	1.0	81.2
rpoB-trnC-GCA_p2	CCCATTTTCATCTCCGAGCA	GGGTTTTTGTTGATCAGGCG	50.0	50.0	58.0	57.9	1476-1499 bp	1.0	81.2
rpoB-trnC-GCA_p3	CTTCTGTTAAGCCCCGATCC	CGGGTTTTTGTTGATCAGGC	55.0	50.0	58.0	57.9	1556-1579 bp	1.0	81.1
rpoB-trnC-GCA_p4	CTTCTGTTAAGCCCCGATCC	GGGTTTTTGTTGATCAGGCG	55.0	50.0	58.0	57.9	1555-1578 bp	1.0	81.1
rpoB-trnC-GCA_p5	CCCATTTTCATCTCCGAGCA	CCGGATTTGAACTGGGGAAT	50.0	50.0	58.0	57.9	1452-1475 bp	1.0	80.6