Hotspot

trnK-UUU-rps16

LSC · 4333-5169 · 837 bp

Back to Corchoropsis

Pi 0.00884

MarkerSeek score 62.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 837
variable_sites: 10
pi: 0.00884
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.2	58.0	942-965 bp	1.0	81.9
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.2	58.0	941-964 bp	1.0	81.9
trnK-UUU-rps16_p3	TACCGTTGAGTTAGCAACCC	AAAAGGAGCTCAACCCACAG	50.0	50.0	57.5	58.0	928-951 bp	1.0	79.6
trnK-UUU-rps16_p4	AAAGCCGAGTACTCTACCGT	CGGGGGTTTTTACGGAACTT	50.0	50.0	58.2	58.4	895-918 bp	1.0	79.0
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	CGGGGGTTTTTACGGAACTT	50.0	50.0	58.2	58.4	894-917 bp	1.0	79.0