Hotspot

ndhC-trnV-UAC

LSC · 38728-39319 · 592 bp

Back to Corallorhiza

Pi 0.0497

MarkerSeek score 80.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 592
variable_sites: 80
pi: 0.0497
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TGGTTCAGGCATTTGCTCAT	TGCGCCAATGTTTTTCAAGG	45.0	45.0	58.1	58.1	1671-2036 bp	0.769231	80.0
ndhC-trnV-UAC_p2	TGGTTCAGGCATTTGCTCAT	TTGGTTTGATCCGGTTCAGG	45.0	50.0	58.1	58.1	1547-1921 bp	0.692308	76.8
ndhC-trnV-UAC_p3	ACATCAAAGCTCATTGCCCA	TTGGTTTGATCCGGTTCAGG	45.0	50.0	58.1	58.1	1347-1419 bp	0.230769	54.6
ndhC-trnV-UAC_p4	ACATCAAAGCTCATTGCCCA	TGCGCCAATGTTTTTCAAGG	45.0	45.0	58.1	58.1	1471-1534 bp	0.230769	54.3
ndhC-trnV-UAC_p5	ACATCAAAGCTCATTGCCCA	CAGTCCATTCAATGCCAGGT	45.0	50.0	58.1	58.2	1275 bp	0.153846	44.3