Hotspot

trnT-UGU-trnL-UAA

LSC · 34888-35477 · 590 bp

Back to Corallorhiza

Pi 0.0511

MarkerSeek score 79.60

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 590
variable_sites: 92
pi: 0.0511
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	AGAAAAAGAGCCGGCTATCG	GAGTCTCTGCACCTATCCCT	50.0	55.0	58.1	57.9	802-871 bp	1.0	90.9
trnT-UGU-trnL-UAA_p2	AGAAAAAGAGCCGGCTATCG	GCTTCCATTGAGTCTCTGCA	50.0	50.0	58.1	57.9	811-880 bp	1.0	90.7
trnT-UGU-trnL-UAA_p3	TAAAACCTGGCCTGTTGACC	GAGTCTCTGCACCTATCCCT	50.0	55.0	58.0	57.9	1379-1448 bp	0.923077	88.2
trnT-UGU-trnL-UAA_p4	TAAAACCTGGCCTGTTGACC	GCTTCCATTGAGTCTCTGCA	50.0	50.0	58.0	57.9	1388-1457 bp	0.923077	88.0
trnT-UGU-trnL-UAA_p5	TAAAACCTGGCCTGTTGACC	CCGTAGCGTCTACCAATTCC	50.0	55.0	58.0	58.2	1261-1262 bp	0.307692	54.6