Hotspot

matK-trnK-UUU

LSC · 2806-3830 · 1025 bp

Back to Corallorhiza

Pi 0.0418

MarkerSeek score 79.50

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 1025
variable_sites: 128
pi: 0.0418
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	AGAATTCTTGTGCCCCCAAG	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.1	57.9	1888-2093 bp	0.692308	78.0
matK-trnK-UUU_p2	GAATTCTTGTGCCCCCAAGA	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.1	57.9	1887-2092 bp	0.692308	78.0
matK-trnK-UUU_p3	AGAATTCTTGTGCCCCCAAG	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.1	58.2	1258-1373 bp	0.692308	77.8
matK-trnK-UUU_p4	AGAATTCTTGTGCCCCCAAG	ATCCGGGTTGCTAACTCAAC	50.0	50.0	58.1	57.9	1276-1391 bp	0.461538	67.1
matK-trnK-UUU_p5	GAATTCTTGTGCCCCCAAGA	ATCCGGGTTGCTAACTCAAC	50.0	50.0	58.1	57.9	1275-1390 bp	0.461538	67.0