Hotspot

ycf80

Genome · 147603-148958 · 1356 bp

Back to Corallina

Pi 0.045

MarkerSeek score 69.70

Primer available yes

Label ycf80

Feature Metadata

label_name: ycf80
feature_type: gene
region: Genome
length_bp: 1356
variable_sites: 61
pi: 0.045
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf80_p1	ACAACTGGTGAACCATGGAC	ATCCCACCAGCTCCATAAGT	50.0	50.0	58.0	58.1	1881 bp	1.0	86.2
ycf80_p2	CAACTGGTGAACCATGGACA	ATCCCACCAGCTCCATAAGT	50.0	50.0	58.0	58.1	1880 bp	1.0	86.2
ycf80_p3	GGCGAGCATGGTCAAAAATC	ATCCCACCAGCTCCATAAGT	50.0	50.0	58.1	58.1	2111 bp	1.0	85.6
ycf80_p4	TGGTATTAACGAGCGTCAGC	ATCCCACCAGCTCCATAAGT	50.0	50.0	58.1	58.1	2031 bp	0.666667	69.2
ycf80_p5	TCGCTATAGAGGACCACGTT	ATCCCACCAGCTCCATAAGT	50.0	50.0	58.0	58.1	2202 bp	0.333333	52.1