Hotspot

upp

Genome · 32112-32699 · 588 bp

Back to Corallina

Pi 0.0567

MarkerSeek score 76.60

Primer available yes

Label upp

Feature Metadata

label_name: upp
feature_type: gene
region: Genome
length_bp: 588
variable_sites: 49
pi: 0.0567
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
upp_p1	AACCATGGCAAGAAAGACGT	CGTCCCTACGTTTTCTGCTA	45.0	50.0	58.0	57.4	1795 bp	1.0	79.1
upp_p2	AAGAAAGACGTGCTGCTGAA	CGTCCCTACGTTTTCTGCTA	45.0	50.0	58.0	57.4	1786 bp	1.0	78.9
upp_p3	CACCTCGCTCATGGTTAACA	CGTCCCTACGTTTTCTGCTA	50.0	50.0	57.9	57.4	1687 bp	1.0	78.6
upp_p4	ATCAGCACAAGGACCAACTG	CGTCCCTACGTTTTCTGCTA	50.0	50.0	58.1	57.4	1962 bp	0.333333	47.4
upp_p5	CATCAGCACAAGGACCAACT	CGTCCCTACGTTTTCTGCTA	50.0	50.0	58.1	57.4	1963 bp	0.333333	47.4