Hotspot

ORF700

Genome · 135111-137213 · 2103 bp

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Pi 0.0428

MarkerSeek score 71.60

Primer available yes

Label ORF700

Feature Metadata

label_name: ORF700
feature_type: gene
region: Genome
length_bp: 2103
variable_sites: 134
pi: 0.0428
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF700_p1	ACGATCCATCAAGAAACCCT	AGCCCAACTTTATCTAGTGGC	45.0	47.6	56.5	57.7	2883 bp	0.666667	47.3
ORF700_p2	AACGATCCATCAAGAAACCCT	AGCCCAACTTTATCTAGTGGC	42.9	47.6	57.3	57.7	2884 bp	0.666667	45.4
ORF700_p3	ACGATCCATCAAGAAACCCT	CCCAACTTTATCTAGTGGCGA	45.0	47.6	56.5	57.5	2881 bp	0.666667	45.3
ORF700_p4	AACGATCCATCAAGAAACCCT	CCCAACTTTATCTAGTGGCGA	42.9	47.6	57.3	57.5	2882 bp	0.666667	43.3
ORF700_p5	ACGATCCATCAAGAAACCCT	GCCCAACTTTATCTAGTGGC	45.0	50.0	56.5	56.2	2882 bp	0.666667	43.1