Hotspot

ORF456

Genome · 91459-92826 · 1368 bp

Back to Corallina

Pi 0.0482
MarkerSeek score 76.20
Primer available yes
Label ORF456

Feature Metadata

label_name
ORF456
feature_type
gene
region
Genome
length_bp
1368
variable_sites
97
pi
0.0482
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ORF456_p1 GATGTATGCAGGACCAGCTC ACCCGAGACTAACACATCCA 55.0 50.0 58.1 58.1 2457-2466 bp 0.666667 67.6
ORF456_p2 GATGTATGCAGGACCAGCTC AACACTAGCAGTAACAGGCG 55.0 50.0 58.1 57.9 2108-2117 bp 0.666667 67.5
ORF456_p3 TGCAGGACCAGCTCATATTG ACCCGAGACTAACACATCCA 50.0 50.0 57.7 58.1 2451-2460 bp 0.666667 65.9
ORF456_p4 GATGTATGCAGGACCAGCTC TTAAGACCACCAAATCGGGC 55.0 50.0 58.1 58.2 2205 bp 0.333333 51.0
ORF456_p5 GATGTATGCAGGACCAGCTC ACGTTTTATCAGCTGCCTGT 55.0 45.0 58.1 57.8 2346 bp 0.333333 50.9