Hotspot

ORF172

Genome · 33893-34444 · 552 bp

Back to Corallina

Pi 0.0543

MarkerSeek score 75.70

Primer available yes

Label ORF172

Feature Metadata

label_name: ORF172
feature_type: gene
region: Genome
length_bp: 552
variable_sites: 44
pi: 0.0543
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF172_p1	CGTAACGATGCTTGGGTACA	TGCTATGGGGTACGATGCTA	50.0	50.0	58.0	58.0	983 bp	1.0	85.0
ORF172_p2	CGTAACGATGCTTGGGTACA	TTTGCTATGGGGTACGATGC	50.0	50.0	58.0	58.0	985 bp	1.0	84.7
ORF172_p3	CGTAGGGACGGAAAAGCTTT	TGCTATGGGGTACGATGCTA	50.0	50.0	58.2	58.0	1281 bp	1.0	82.7
ORF172_p4	CGTAACGATGCTTGGGTACA	CCGGTTTTGCGGATAGAAGA	50.0	50.0	58.0	58.0	1572 bp	0.666667	69.6
ORF172_p5	CGTAACGATGCTTGGGTACA	GGCCGGCGCTTTAATTATTC	50.0	50.0	58.0	58.0	1094 bp	0.333333	53.0