Hotspot

trnV(tac)-trnR3(tct)

Genome · 176948-176970 · 23 bp

Back to Corallina

Pi 0.174

MarkerSeek score 78.40

Primer available yes

Label trnV(tac)-trnR3(tct)

Feature Metadata

label_name: trnV(tac)-trnR3(tct)
feature_type: igs
region: Genome
length_bp: 23
variable_sites: 4
pi: 0.174
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV(tac)-trnR3(tct)_p1	TGGTTCAAATCCAGTACCGC	AAGGTCCCTGTCCTAATCCC	50.0	55.0	58.2	58.1	81 bp	1.0	85.6
trnV(tac)-trnR3(tct)_p2	CTTACAAGCAGATGGCCACT	GGTCCCTGTCCTAATCCCTT	50.0	55.0	57.9	58.1	98 bp	1.0	85.5
trnV(tac)-trnR3(tct)_p3	CTTACAAGCAGATGGCCACT	AAGGTCCCTGTCCTAATCCC	50.0	55.0	57.9	58.1	100 bp	1.0	85.4
trnV(tac)-trnR3(tct)_p4	TTACAAGCAGATGGCCACTG	GGTCCCTGTCCTAATCCCTT	50.0	55.0	58.2	58.1	97 bp	1.0	85.1
trnV(tac)-trnR3(tct)_p5	TTACAAGCAGATGGCCACTG	AAGGTCCCTGTCCTAATCCC	50.0	55.0	58.2	58.1	99 bp	1.0	85.0