Hotspot

trnS(gct)-trnD(gtc)

Genome · 58086-58143 · 58 bp

Back to Corallina

Pi 0.0805

MarkerSeek score 78.30

Primer available yes

Label trnS(gct)-trnD(gtc)

Feature Metadata

label_name: trnS(gct)-trnD(gtc)
feature_type: igs
region: Genome
length_bp: 58
variable_sites: 7
pi: 0.0805
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS(gct)-trnD(gtc)_p1	GAGAGATGGCTGAGTGGTTG	GGGCGATGCTCTAACCAATT	55.0	50.0	58.0	58.3	181 bp	1.0	80.4
trnS(gct)-trnD(gtc)_p2	TGAAAGCGGCAGATTGCTAA	GGGCGATGCTCTAACCAATT	45.0	50.0	58.2	58.3	163 bp	1.0	79.4
trnS(gct)-trnD(gtc)_p3	TTGAAAGCGGCAGATTGCTA	GGGCGATGCTCTAACCAATT	45.0	50.0	58.2	58.3	164 bp	1.0	79.4
trnS(gct)-trnD(gtc)_p4	GAGAGATGGCTGAGTGGTTG	ACCAATTGAACTACGGTCCC	55.0	50.0	58.0	57.5	168 bp	1.0	79.3
trnS(gct)-trnD(gtc)_p5	TGAAAGCGGCAGATTGCTAA	ACCAATTGAACTACGGTCCC	45.0	50.0	58.2	57.5	150 bp	1.0	78.4