Hotspot

trnM1(cat)-trnS(gct)

Genome · 57944-57994 · 51 bp

Back to Corallina

Pi 0.085

MarkerSeek score 84.80

Primer available yes

Label trnM1(cat)-trnS(gct)

Feature Metadata

label_name: trnM1(cat)-trnS(gct)
feature_type: igs
region: Genome
length_bp: 51
variable_sites: 6
pi: 0.085
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM1(cat)-trnS(gct)_p1	AGGTTCAAATCCCGCTTGAG	CAACCACTCAGCCATCTCTC	50.0	55.0	58.2	58.0	91-95 bp	1.0	87.0
trnM1(cat)-trnS(gct)_p2	GACTCATAAGCCCAAGGTCG	CAACCACTCAGCCATCTCTC	55.0	55.0	58.1	58.0	112-116 bp	1.0	86.3
trnM1(cat)-trnS(gct)_p3	AGGTTCAAATCCCGCTTGAG	TAGCAATCTGCCGCTTTCAA	50.0	45.0	58.2	58.2	108-112 bp	1.0	84.4
trnM1(cat)-trnS(gct)_p4	GACTCATAAGCCCAAGGTCG	TAGCAATCTGCCGCTTTCAA	55.0	45.0	58.1	58.2	129-133 bp	1.0	84.2
trnM1(cat)-trnS(gct)_p5	GACTCATAAGCCCAAGGTCG	TTAGCAATCTGCCGCTTTCA	55.0	45.0	58.1	58.2	130-134 bp	1.0	84.1