Hotspot

argB-trnM1(cat)

Genome · 57524-57869 · 346 bp

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Pi 0.0922
MarkerSeek score 79.50
Primer available yes
Label argB-trnM1(cat)

Feature Metadata

label_name
argB-trnM1(cat)
feature_type
igs
region
Genome
length_bp
346
variable_sites
47
pi
0.0922
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
argB-trnM1(cat)_p1 TGGAATAGGTTCAATGCTAGT CGACCTTGGGCTTATGAGTC 38.1 55.0 54.3 58.1 415-421 bp 1.0 51.2
argB-trnM1(cat)_p2 TGGAATAGGTTCAATGCTAGT CTCAAGCGGGATTTGAACCT 38.1 50.0 54.3 58.2 436-442 bp 1.0 50.3
argB-trnM1(cat)_p3 TGGAATAGGTTCAATGCTAGT TTTCTAGATGGCTCAAGCGG 38.1 50.0 54.3 57.7 447-453 bp 1.0 49.1
argB-trnM1(cat)_p4 ATGGAATAGGTTCAATGCTAGT CGACCTTGGGCTTATGAGTC 36.4 55.0 54.9 58.1 416-422 bp 1.0 49.1
argB-trnM1(cat)_p5 TGGAATAGGTTCAATGCTAGT GGATTTGAACCTGCGACCTT 38.1 50.0 54.3 58.5 428-434 bp 1.0 49.1