Hotspot

clpC-rpl19

Genome · 53251-53774 · 524 bp

Back to Corallina

Pi 0.077

MarkerSeek score 83.30

Primer available yes

Label clpC-rpl19

Feature Metadata

label_name: clpC-rpl19
feature_type: igs
region: Genome
length_bp: 524
variable_sites: 57
pi: 0.077
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpC-rpl19_p1	TTGTGTCCTAAACGCCTAGC	ATTGCCAGCGGATTTACAGT	50.0	45.0	57.9	57.9	1125-1181 bp	1.0	88.1
clpC-rpl19_p2	AAACGCCTAGCTTCTTCCTG	ATTGCCAGCGGATTTACAGT	50.0	45.0	57.9	57.9	1116-1172 bp	1.0	87.9
clpC-rpl19_p3	TGAGCTGCAATACCTGTTCC	ATTGCCAGCGGATTTACAGT	50.0	45.0	57.9	57.9	1188-1244 bp	1.0	87.4
clpC-rpl19_p4	ACGAGCTTGCATGTTACCAT	ATTGCCAGCGGATTTACAGT	45.0	45.0	57.9	57.9	1525-1581 bp	1.0	85.9
clpC-rpl19_p5	GGCTCACCGATCAATACAGG	ATTGCCAGCGGATTTACAGT	55.0	45.0	58.1	57.9	1726-1739 bp	0.666667	68.3