Hotspot

moeB-trnS(gga)

Genome · 48100-48111 · 12 bp

Back to Corallina

Pi 0.167

MarkerSeek score 77.50

Primer available yes

Label moeB-trnS(gga)

Feature Metadata

label_name: moeB-trnS(gga)
feature_type: igs
region: Genome
length_bp: 12
variable_sites: 3
pi: 0.167
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
moeB-trnS(gga)_p1	ACAGGACTTGTTCAAGCTACA	GGTGGCTGAGTGGTCTAAAG	42.9	55.0	57.2	57.9	562-564 bp	1.0	69.0
moeB-trnS(gga)_p2	CAGGACTTGTTCAAGCTACAGA	GGTGGCTGAGTGGTCTAAAG	45.5	55.0	58.0	57.9	561-563 bp	1.0	67.7
moeB-trnS(gga)_p3	ACAGGACTTGTTCAAGCTACA	AGGTGGCTGAGTGGTCTAAA	42.9	50.0	57.2	58.3	563-565 bp	1.0	67.7
moeB-trnS(gga)_p4	ACAGGACTTGTTCAAGCTACA	ACTGCCAAAAATCTGTCGGT	42.9	45.0	57.2	58.0	928 bp	0.666667	55.3
moeB-trnS(gga)_p5	CAGGACTTGTTCAAGCTACAGA	ACTGCCAAAAATCTGTCGGT	45.5	45.0	58.0	58.0	927 bp	0.666667	54.0