Hotspot

groEL-trnR1(acg)

Genome · 36020-36658 · 639 bp

Back to Corallina

Pi 0.0777

MarkerSeek score 86.20

Primer available yes

Label groEL-trnR1(acg)

Feature Metadata

label_name: groEL-trnR1(acg)
feature_type: igs
region: Genome
length_bp: 639
variable_sites: 65
pi: 0.0777
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
groEL-trnR1(acg)_p1	TCCATACCTTTTTCTAAAGCTTTCC	TTTTTATCGGGCAAGGAGGG	36.0	50.0	57.5	57.9	628-779 bp	1.0	51.4
groEL-trnR1(acg)_p2	CCATACCTTTTTCTAAAGCTTTCCT	TTTTTATCGGGCAAGGAGGG	36.0	50.0	57.5	57.9	627-778 bp	1.0	51.4
groEL-trnR1(acg)_p3	ACATCCATACCTTTTTCTAAAGCT	TTTTTATCGGGCAAGGAGGG	33.3	50.0	56.3	57.9	631-782 bp	1.0	51.4
groEL-trnR1(acg)_p4	CCATACCTTTTTCTAAAGCTTTCC	TTTTTATCGGGCAAGGAGGG	37.5	50.0	56.2	57.9	627-778 bp	1.0	51.4
groEL-trnR1(acg)_p5	ACCTTTTTCTAAAGCTTTCCT	TTTTTATCGGGCAAGGAGGG	33.3	50.0	53.2	57.9	623-774 bp	1.0	51.4