Hotspot

rrl-trnA(tgc)

Genome · 26264-26313 · 50 bp

Back to Corallina

Pi 0.0533

MarkerSeek score 79.40

Primer available yes

Label rrl-trnA(tgc)

Feature Metadata

label_name: rrl-trnA(tgc)
feature_type: igs
region: Genome
length_bp: 50
variable_sites: 4
pi: 0.0533
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrl-trnA(tgc)_p1	TCATCGCTTCTGAATGCCAA	GGTTCGAGTCCGCTTATCTC	45.0	55.0	57.9	57.9	120-122 bp	1.0	82.1
rrl-trnA(tgc)_p2	TCATCGCTTCTGAATGCCAA	GTTCGAGTCCGCTTATCTCC	45.0	55.0	57.9	57.9	119-121 bp	1.0	82.1
rrl-trnA(tgc)_p3	TTCATCGCTTCTGAATGCCA	GTTCGAGTCCGCTTATCTCC	45.0	55.0	57.9	57.9	120-122 bp	1.0	82.1
rrl-trnA(tgc)_p4	TTCATCGCTTCTGAATGCCA	GGTTCGAGTCCGCTTATCTC	45.0	55.0	57.9	57.9	121-123 bp	1.0	82.0
rrl-trnA(tgc)_p5	TCATCGCTTCTGAATGCCAA	CAGTTGGTAGAGTGCTGCTT	45.0	50.0	57.9	57.8	159-161 bp	1.0	80.7