Hotspot

trnN(gtt)-trnL(tag)

Genome · 4798-4903 · 106 bp

Back to Corallina

Pi 0.0692

MarkerSeek score 78.20

Primer available yes

Label trnN(gtt)-trnL(tag)

Feature Metadata

label_name: trnN(gtt)-trnL(tag)
feature_type: igs
region: Genome
length_bp: 106
variable_sites: 11
pi: 0.0692
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN(gtt)-trnL(tag)_p1	CCGATTGCTCTACCACTGAG	TACCAATTTCACCACGTCCG	55.0	50.0	57.8	58.2	157 bp	1.0	82.3
trnN(gtt)-trnL(tag)_p2	CCCCAGGAAGGATTTGAACC	TACCAATTTCACCACGTCCG	55.0	50.0	58.1	58.2	197 bp	1.0	81.7
trnN(gtt)-trnL(tag)_p3	CCGATTGCTCTACCACTGAG	GACGGAGAGACTCGAACTCT	55.0	55.0	57.8	58.3	215 bp	1.0	80.2
trnN(gtt)-trnL(tag)_p4	CCCCAGGAAGGATTTGAACC	GACGGAGAGACTCGAACTCT	55.0	55.0	58.1	58.3	255 bp	1.0	80.1
trnN(gtt)-trnL(tag)_p5	TCTCCCCAGGAAGGATTTGA	TACCAATTTCACCACGTCCG	50.0	50.0	57.6	58.2	200 bp	1.0	79.9