Hotspot

rps15-trnN-GUU

SSC · 123995-130438 · 6444 bp

Back to Coptis

Pi 0.014
MarkerSeek score 55.80
Primer available yes
Label rps15-trnN-GUU

Feature Metadata

label_name
rps15-trnN-GUU
feature_type
igs
region
SSC
length_bp
6444
variable_sites
280
pi
0.014
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-trnN-GUU_p1 CTCTACGACGTCTGGGAGAT AGCCGACGCCAACATTAATA 55.0 45.0 58.0 57.7 1013-1165 bp 0.625 68.6
rps15-trnN-GUU_p2 GTTTTGCGCAGTTGATCCAA AGCCGACGCCAACATTAATA 45.0 45.0 57.9 57.7 418-570 bp 0.625 68.1
rps15-trnN-GUU_p3 CCGCTGCTATGCTGTTCTTA AGCCGACGCCAACATTAATA 50.0 45.0 58.1 57.7 584-694 bp 0.5 63.6
rps15-trnN-GUU_p4 CCCGCGAAATAGCTATGGTT AGCCGACGCCAACATTAATA 50.0 45.0 58.1 57.7 842-994 bp 0.5 62.7
rps15-trnN-GUU_p5 ATGGATAGCCGAACCGTTTC AGCCGACGCCAACATTAATA 50.0 45.0 58.1 57.7 944 bp 0.125 42.0