Hotspot

ndhE-ndhG

SSC · 118785-119033 · 249 bp

Back to Coptis

Pi 0.0205
MarkerSeek score 65.10
Primer available yes
Label ndhE-ndhG

Feature Metadata

label_name
ndhE-ndhG
feature_type
igs
region
SSC
length_bp
249
variable_sites
17
pi
0.0205
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhE-ndhG_p1 ACAAGTACATGTTCGAGCATCA GCCTTGATAGGTGCAATTGC 40.9 50.0 58.1 57.8 310-328 bp 1.0 70.0
ndhE-ndhG_p2 ACAAGTACATGTTCGAGCATCA TGCTATGGCTCGTCAGTACT 40.9 50.0 58.1 58.2 293-311 bp 1.0 70.0
ndhE-ndhG_p3 ACAAGTACATGTTCGAGCATCA TTGCTATGGCTCGTCAGTAC 40.9 50.0 58.1 57.4 294-312 bp 1.0 67.3
ndhE-ndhG_p4 ACAAGTACATGTTCGAGCATCA AGGTGCAATTGCTATGGCTC 40.9 50.0 58.1 58.6 302-320 bp 1.0 67.0
ndhE-ndhG_p5 ACAAGTACATGTTCGAGCATCA AGTTGCCTTGATAGGTGCAA 40.9 45.0 58.1 57.4 314-332 bp 1.0 66.9