Hotspot

ndhD-psaC

SSC · 117892-117988 · 97 bp

Back to Coptis

Pi 0.0657
MarkerSeek score 80.30
Primer available yes
Label ndhD-psaC

Feature Metadata

label_name
ndhD-psaC
feature_type
igs
region
SSC
length_bp
97
variable_sites
17
pi
0.0657
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-psaC_p1 TCGTGGTAAAGGCAAGATACA GCATGAGACAACTCGTAGCA 42.9 50.0 57.0 58.0 165-177 bp 1.0 73.2
ndhD-psaC_p2 CGTGGTAAAGGCAAGATACAC GCATGAGACAACTCGTAGCA 47.6 50.0 57.0 58.0 164-176 bp 1.0 72.8
ndhD-psaC_p3 AGGCAAGATACACTTTGACCA GCATGAGACAACTCGTAGCA 42.9 50.0 57.2 58.0 156-163 bp 0.875 69.7
ndhD-psaC_p4 AGGCAAGATACACTTTGACCA GAGACAACTCGTAGCATGGG 42.9 55.0 57.2 58.1 152-159 bp 0.875 69.2
ndhD-psaC_p5 AGGCAAGATACACTTTGACCA TCGTAGCATGGGTCTAGCTT 42.9 50.0 57.2 58.2 144-151 bp 0.875 68.0