Hotspot

ccsA-ndhD

SSC · 116075-116361 · 287 bp

Back to Coptis

Pi 0.0225
MarkerSeek score 65.00
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
287
variable_sites
21
pi
0.0225
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGGTTCATTCGCATTAACATCT GACAAGGTAGAGGCTATTATATCT 36.4 37.5 56.4 54.8 327-357 bp 1.0 48.8
ccsA-ndhD_p2 TGGTTCATTCGCATTAACATCT ACAAGGTAGAGGCTATTATATCT 36.4 34.8 56.4 53.6 326-356 bp 1.0 48.8
ccsA-ndhD_p3 TGGTTCATTCGCATTAACATCT GACAAGGTAGAGGCTATTATATC 36.4 39.1 56.4 53.4 327-357 bp 1.0 48.8
ccsA-ndhD_p4 AGGGCTACATAGTTATGGTTCA GACAAGGTAGAGGCTATTATATCT 40.9 37.5 56.3 54.8 342-372 bp 1.0 48.6
ccsA-ndhD_p5 AGGGCTACATAGTTATGGTTCA ACAAGGTAGAGGCTATTATATCT 40.9 34.8 56.3 53.6 341-371 bp 1.0 48.6