Hotspot

ycf1-rpl32

SSC · 111337-114132 · 2796 bp

Back to Coptis

Pi 0.0119
MarkerSeek score 54.10
Primer available yes
Label ycf1-rpl32

Feature Metadata

label_name
ycf1-rpl32
feature_type
igs
region
SSC
length_bp
2796
variable_sites
104
pi
0.0119
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-rpl32_p1 GCCGTGCACAAAAACTATCC AGTACGTTTCTTCGGAACCG 50.0 50.0 58.0 57.9 2941-2962 bp 0.375 55.7
ycf1-rpl32_p2 TCTGCCGTGCACAAAAACTA AGTACGTTTCTTCGGAACCG 45.0 50.0 58.3 57.9 2944-2965 bp 0.375 53.2
ycf1-rpl32_p3 TTTCTGCCGTGCACAAAAAC AGTACGTTTCTTCGGAACCG 45.0 50.0 58.4 57.9 2946-2967 bp 0.375 52.9
ycf1-rpl32_p4 ACCCACCTAGTTTGTCAACG AGTACGTTTCTTCGGAACCG 50.0 50.0 57.8 57.9 2995-2999 bp 0.25 48.4
ycf1-rpl32_p5 CCCACCTAGTTTGTCAACGT AGTACGTTTCTTCGGAACCG 50.0 50.0 57.8 57.9 2994-2998 bp 0.25 48.4