Hotspot

rpl2-rpl23

IRb · 86241-86246 · 6 bp

Back to Coptis

Pi 0.0417
MarkerSeek score 67.30
Primer available yes
Label rpl2-rpl23

Feature Metadata

label_name
rpl2-rpl23
feature_type
igs
region
IRb
length_bp
6
variable_sites
1
pi
0.0417
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2-rpl23_p1 TGCTCGGGGTAGAAGTTTTG CGCTTCAACCGGGTTATTCT 50.0 50.0 57.8 58.3 105 bp 1.0 77.2
rpl2-rpl23_p2 GCTCGGGGTAGAAGTTTTGT CGCTTCAACCGGGTTATTCT 50.0 50.0 57.8 58.3 104 bp 1.0 77.2
rpl2-rpl23_p3 GTGCTCGGGGTAGAAGTTTT CGCTTCAACCGGGTTATTCT 50.0 50.0 57.8 58.3 106 bp 1.0 77.2
rpl2-rpl23_p4 GGATTTCACTTGACTGCCGA CGCTTCAACCGGGTTATTCT 50.0 50.0 58.2 58.3 140 bp 1.0 76.9
rpl2-rpl23_p5 TGCTCGGGGTAGAAGTTTTG ATCATTACGCTTCAACCGGG 50.0 50.0 57.8 58.3 112 bp 1.0 76.6