Hotspot

psbT-psbN

LSC · 74665-74738 · 74 bp

Back to Coptis

Pi 0.0574
MarkerSeek score 63.40
Primer available yes
Label psbT-psbN

Feature Metadata

label_name
psbT-psbN
feature_type
igs
region
LSC
length_bp
74
variable_sites
9
pi
0.0574
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbT-psbN_p1 TTTTCCGAGAACCACCCAAG GATCCATTCGAGGAACACGG 50.0 55.0 58.0 58.4 145 bp 1.0 81.3
psbT-psbN_p2 AGGTTCCGACTAAAAAGGCG GATCCATTCGAGGAACACGG 50.0 55.0 58.2 58.4 127 bp 1.0 80.7
psbT-psbN_p3 GGTTCCGACTAAAAAGGCGA GATCCATTCGAGGAACACGG 50.0 55.0 58.2 58.4 126 bp 1.0 80.7
psbT-psbN_p4 TTTTCCGAGAACCACCCAAG AGATCCATTCGAGGAACACG 50.0 50.0 58.0 57.4 146 bp 1.0 80.0
psbT-psbN_p5 TTTTCCGAGAACCACCCAAG GGCAACCCTCTCAACAACTA 50.0 50.0 58.0 57.4 168 bp 1.0 79.6