Hotspot

trnL-CAA-ycf2

IRa · 147171-147773 · 603 bp

Back to Copernicia

Pi 0.0381

MarkerSeek score 83.00

Primer available yes

Label trnL-CAA-ycf2

Feature Metadata

label_name: trnL-CAA-ycf2
feature_type: igs
region: IRa
length_bp: 603
variable_sites: 34
pi: 0.0381
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	AAATGGTAGACACGCGAGAC	AAAGAGATGGCTTTTCCCGG	50.0	50.0	58.0	58.2	745-1171 bp	1.0	81.5
trnL-CAA-ycf2_p2	TGAAATGGTAGACACGCGAG	AAAGAGATGGCTTTTCCCGG	50.0	50.0	58.0	58.2	747-1173 bp	1.0	81.5
trnL-CAA-ycf2_p3	AAATGGTAGACACGCGAGAC	ATGGCTTTTCCCGGATGAAA	50.0	45.0	58.0	57.8	739-1165 bp	1.0	81.0
trnL-CAA-ycf2_p4	AAATGGTAGACACGCGAGAC	TGGCTTTTCCCGGATGAAAT	50.0	45.0	58.0	57.8	738-1164 bp	1.0	81.0
trnL-CAA-ycf2_p5	TGAAATGGTAGACACGCGAG	TGGCTTTTCCCGGATGAAAT	50.0	45.0	58.0	57.8	740-1166 bp	1.0	81.0