Hotspot

atpB-rbcL

LSC · 56844-57613 · 770 bp

Back to Copernicia

Pi 0.00606

MarkerSeek score 63.10

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 770
variable_sites: 7
pi: 0.00606
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGGGAAAAACGACATCCAGT	GGTGACATAAGTCCCTCCCT	50.0	55.0	58.1	58.1	905-909 bp	1.0	79.2
atpB-rbcL_p2	TGGGCCAATAATTTGAGCGA	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.9	58.1	883-887 bp	1.0	78.9
atpB-rbcL_p3	GGGGAAAAACGACATCCAGT	TGGTGACATAAGTCCCTCCC	50.0	55.0	58.1	58.4	906-910 bp	1.0	76.8
atpB-rbcL_p4	TGGGCCAATAATTTGAGCGA	TGGTGACATAAGTCCCTCCC	45.0	55.0	57.9	58.4	884-888 bp	1.0	76.5
atpB-rbcL_p5	CTGGGCCAATAATTTGAGCG	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.5	58.1	884-888 bp	1.0	76.0