Hotspot

rpl16

LSC · 82635-84070 · 1436 bp

Back to Convallaria

Pi 0.00186

MarkerSeek score 60.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1436
variable_sites: 4
pi: 0.00186
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AAATAAACCCGCGGTTGTTT	TCCTTTTTCCGTTAAATCAAACA	40.0	30.4	56.8	55.3	1596-1598 bp	1.0	40.3
rpl16_p2	AATAAACCCGCGGTTGTTTT	TCCTTTTTCCGTTAAATCAAACA	40.0	30.4	56.8	55.3	1595-1597 bp	1.0	40.3
rpl16_p3	ATAAACCCGCGGTTGTTTTT	TCCTTTTTCCGTTAAATCAAACA	40.0	30.4	56.8	55.3	1594-1596 bp	1.0	40.3
rpl16_p4	AAATAAACCCGCGGTTGTTTT	TCCTTTTTCCGTTAAATCAAACA	38.1	30.4	57.5	55.3	1596-1598 bp	1.0	40.3
rpl16_p5	AATAAACCCGCGGTTGTTTTT	TCCTTTTTCCGTTAAATCAAACA	38.1	30.4	57.5	55.3	1595-1597 bp	1.0	40.3