Hotspot

ccsA

SSC · 118876-119871 · 996 bp

Back to Convallaria

Pi 0

MarkerSeek score 25.80

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 996
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	TTCTCGATGGCTTCTCTTGC	50.0	50.0	58.0	58.0	1715 bp	1.0	80.4
ccsA_p2	TGAAATCGGTAGACACGCTG	TTCTCGATGGCTTCTCTTGC	50.0	50.0	58.0	58.0	1714 bp	1.0	80.4
ccsA_p3	GAGTGGCGGCATACTGTAAA	TTCTCGATGGCTTCTCTTGC	50.0	50.0	58.0	58.0	1655 bp	1.0	80.3
ccsA_p4	GTGAAATCGGTAGACACGCT	CTCGATGGCTTCTCTTGCAT	50.0	50.0	58.0	58.0	1713 bp	1.0	80.1
ccsA_p5	TGAAATCGGTAGACACGCTG	CTCGATGGCTTCTCTTGCAT	50.0	50.0	58.0	58.0	1712 bp	1.0	80.1