Hotspot

atpB

LSC · 54102-55589 · 1488 bp

Back to Convallaria

Pi 0.00314

MarkerSeek score 60.70

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1488
variable_sites: 7
pi: 0.00314
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	CGTGGTTTGGTAATACGCCA	CGATCAACTTGCTATCGGACA	50.0	47.6	58.6	58.2	1685-1686 bp	1.0	66.4
atpB_p2	GGGGCGTGGTTTGGTAATAC	CGATCAACTTGCTATCGGACA	55.0	47.6	58.9	58.2	1689-1690 bp	1.0	63.6
atpB_p3	CGTGGTTTGGTAATACGCCA	CGATCAACTTGCTATCGGAC	50.0	50.0	58.6	56.4	1685-1686 bp	1.0	63.4
atpB_p4	AGGGGCGTGGTTTGGTAATA	CGATCAACTTGCTATCGGACA	50.0	47.6	59.0	58.2	1690-1691 bp	1.0	62.8
atpB_p5	TAGGGGCGTGGTTTGGTAAT	CGATCAACTTGCTATCGGACA	50.0	47.6	59.0	58.2	1691-1692 bp	1.0	62.8