Hotspot

rpl10-ycf2

IRa · 151494-152726 · 1233 bp

Back to Convallaria

Pi 0.00703

MarkerSeek score 51.30

Primer available yes

Label rpl10-ycf2

Feature Metadata

label_name: rpl10-ycf2
feature_type: igs
region: IRa
length_bp: 1233
variable_sites: 13
pi: 0.00703
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl10-ycf2_p1	CTCCATGGACCAAGATCTGC	AAAGAGATGGCTTTTCCCGG	55.0	50.0	58.0	58.2	1407-1429 bp	1.0	79.5
rpl10-ycf2_p2	CTCCATGGACCAAGATCTGC	ATGGCTTTTCCCGGATGAAA	55.0	45.0	58.0	57.8	1401-1423 bp	1.0	79.0
rpl10-ycf2_p3	CTCCATGGACCAAGATCTGC	TGGCTTTTCCCGGATGAAAT	55.0	45.0	58.0	57.8	1400-1422 bp	1.0	79.0
rpl10-ycf2_p4	CTCCATGGACCAAGATCTGC	GATGGCTTTTCCCGGATGAA	55.0	50.0	58.0	58.2	1402-1424 bp	1.0	78.9
rpl10-ycf2_p5	CTTCTTGGGACTCCATGGAC	AAAGAGATGGCTTTTCCCGG	55.0	50.0	57.6	58.2	1417-1439 bp	1.0	76.5